2FMJ
220-loop mutant of streptomyces griseus trypsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-11-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 58.319, 58.319, 100.337 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.580 - 1.650 |
| Rwork | 0.157 |
| R-free | 0.18900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | XTALVIEW |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.650 |
| Number of reflections | 22158 |
| Completeness [%] | 95.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 298 | 1.8 M ammonium sulphate 10 mM calcium acetate, pH 6.1 200 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
