2FMB
EIAV PROTEASE COMPLEXED WITH AN INHIBITOR LP-130
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-18 |
| Detector | MACSCIENCE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 42.650, 45.360, 56.760 |
| Unit cell angles | 90.00, 110.36, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.143 * |
| Rwork | 0.143 |
| Structure solution method | RIGID BODY REFINEMENT |
| Starting model (for MR) | 1fmb |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.045 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.049 | 0.300 |
| Number of reflections | 8666 | |
| Completeness [%] | 90.9 | 91.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | pH 4.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | DMSO | ||
| 3 | 1 | reservoir | PEG4000 | 20 (%) | |
| 4 | 1 | reservoir | sodium acetate | 25 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 0.45 (M) | |
| 6 | 1 | reservoir | DMSO | 6 (%) | |
| 7 | 1 | reservoir | sodium azide | 0.1 (%) |
