2FL3
Binary Complex of Restriction Endonuclease HinP1I with Cognate DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-16 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 100.855, 100.855, 139.406 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.170 - 2.390 |
R-factor | 0.249 |
Rwork | 0.246 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ynm |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data scaling software | SCALEPACK |
Phasing software | GLRF |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.170 | 2.480 |
High resolution limit [Å] | 2.390 | 2.390 |
Rmerge | 0.079 | |
Number of reflections | 15524 | |
<I/σ(I)> | 15.2 | |
Completeness [%] | 89.0 | 50.2 |
Redundancy | 20.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 289 | 20% PEG1000, 400mM NaCl, 100mM Bis-Tris propane, and 5% ethylene glycol, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG1000 | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | Bis-Tris propane | ||
4 | 1 | 1 | ethylene glycol | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | NaCl | ||
7 | 1 | 2 | PEG1000 | ||
8 | 1 | 2 | H2O |