2FKB
Crystal structure of a putative enzyme (possible Nudix hydrolase) from Escherichia Coli K12
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795, 0.97943, 0.97956 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 89.701, 134.884, 145.246 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.23053 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.519 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.064 | 0.600 |
| Number of reflections | 58739 | |
| <I/σ(I)> | 33 | 2.3 |
| Completeness [%] | 97.6 | 82.4 |
| Redundancy | 8.6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 1.8M Ammonium Sulphate, 0.1M Na Acetate pH4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
