2FK8
Crystal structure of Hma (MmaA4) from Mycobacterium tuberculosis complexed with S-adenosylmethionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979171 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 56.779, 56.779, 199.035 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.18214 |
| Rwork | 0.179 |
| R-free | 0.23889 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fk7 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.539 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 25549 | |
| <I/σ(I)> | 4.8 | 2.9 |
| Completeness [%] | 97.7 | 88.1 |
| Redundancy | 3.9 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 285 | 4-28% PEG 3350, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 285.0K |
