2FK6
Crystal Structure of RNAse Z/tRNA(Thr) complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.540, 42.400, 110.430 |
| Unit cell angles | 90.00, 121.42, 90.00 |
Refinement procedure
| Resolution | 47.120 - 2.900 |
| R-factor | 0.251 |
| Rwork | 0.250 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y44 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.120 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.070 | 0.496 |
| Number of reflections | 15022 | |
| <I/σ(I)> | 30.8 | 2.8 |
| Completeness [%] | 96.5 | 87.6 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 28% PEG MME 2000, 0.1M Na-MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG MME 2000 | ||
| 2 | 1 | 1 | Na-MES | ||
| 3 | 1 | 2 | PEG MME 2000 | ||
| 4 | 1 | 2 | Na-MES |
