2FJT
Adenylyl cyclase class iv from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-09-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.529, 35.548, 71.985 |
| Unit cell angles | 88.89, 82.63, 65.47 |
Refinement procedure
| Resolution | 9.980 - 1.901 |
| R-factor | 0.21608 |
| Rwork | 0.212 |
| R-free | 0.27293 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | orthorhombic crystal form |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.685 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.026 | 0.170 |
| Number of reflections | 20986 | |
| <I/σ(I)> | 15.7 | 3.9 |
| Completeness [%] | 89.3 | 83.9 |
| Redundancy | 2.04 | 2.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 297 | 100 mM Ammonium sulfate, 100 mM Sodium acetate, 14% PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
