2FJI
Crystal structure of the C-terminal domain of the exocyst subunit Sec6p
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793, 0.9792, 0.9679 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 108.295, 63.301, 151.468 |
Unit cell angles | 90.00, 103.89, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.400 |
Rwork | 0.249 |
R-free | 0.28400 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.073 | 0.421 |
Number of reflections | 76939 | |
Completeness [%] | 98.3 | 98.2 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1%-4% PEG 6000, 10% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1%-4% PEG 6000, 10% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |