2FI0
The crystal structure of the conserved domain protein from Streptococcus pneumoniae TIGR4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | H 3 |
| Unit cell lengths | 92.420, 92.420, 41.343 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.230 - 2.100 |
| R-factor | 0.20438 |
| Rwork | 0.203 |
| R-free | 0.23627 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.277 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.057 | 0.155 |
| Number of reflections | 6747 | |
| <I/σ(I)> | 13.77 | 3.2 |
| Completeness [%] | 91.6 | 43.5 |
| Redundancy | 3 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 287 | 2.5 M NaCl, 0.1M Tris-HCl, 0.2M MgCl2, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 287K |
