2FHZ
Molecular Basis of Inhibition of the Ribonuclease Activity in Colicin E5 by Its Cognate Immunity Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2003-03-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 61.790, 73.640, 110.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.150 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.20000 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.296 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.200 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.034 | 0.175 |
| Number of reflections | 82619 | |
| <I/σ(I)> | 20.1 | 9.1 |
| Completeness [%] | 92.7 | 82.8 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 278 | 50 % saturated ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 278 | 50 % saturated ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
