2FHN
Avidin related protein AVR4 (C122S, K109I mutant) in complex with BNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2005-02-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 77.592, 77.592, 109.727 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.300 |
| R-factor | 0.15183 |
| Rwork | 0.151 |
| R-free | 0.16560 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1y55 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.475 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 82848 | |
| <I/σ(I)> | 22 | |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 3. M farmat, 0.1M acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
