2FH9

Structure and dimerization of the kinase domain from yeast Snf1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Wavelength(s)	0.9686, 0.9796, 0.9794
Spacegroup name	P 4 21 2
Unit cell lengths	108.677, 108.677, 61.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.336
Rwork	0.215
R-free	0.26000
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.383
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.02)
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.800
Number of reflections	8763
Completeness [%]	91.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.1M Tris pH 8.5, 0.2M magnesium chloride, 30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.1M Tris pH 8.5, 0.2M magnesium chloride, 30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.1M Tris pH 8.5, 0.2M magnesium chloride, 30% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K