2FGZ
Crystal Structure Analysis of apo pullulanase from Klebsiella pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.71 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.984, 60.214, 132.791 |
| Unit cell angles | 90.00, 112.61, 90.00 |
Refinement procedure
| Resolution | 14.940 - 1.750 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.21400 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | PHASES |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.080 | 0.384 |
| Number of reflections | 108442 | |
| <I/σ(I)> | 10.4 | 6.8 |
| Completeness [%] | 98.6 | 99.7 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 15-25% polyethyleneglycol 6000 in 0.2M magnesium acetate, 0.2M sodium acetate buffer, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
