2FGW

X-RAY STRUCTURES OF FRAGMENTS FROM BINDING AND NONBINDING VERSIONS OF A HUMANIZED ANTI-CD18 ANTIBODY: STRUCTURAL INDICATIONS OF THE KEY ROLE OF VH RESIDUES 59 TO 65

Experimental procedure

Spacegroup name	P 3
Unit cell lengths	101.000, 101.000, 45.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 3.000
R-factor	0.176
Rwork	0.176
RMSD bond length	0.017
RMSD bond angle	3.600
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Outer shell
High resolution limit [Å]	2.990 *
Rmerge	0.235 *
Number of reflections	885 *
Completeness [%]	85 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	calcium acetate	0.2 (M)
3	1	reservoir	sodium cacodylate	0.1 (M)
4	1	reservoir	PEG8000	9 (%(w/v))