2FGP
Crystal structure of a minimal, all RNA hairpin ribozyme with modifications (g8dap, u39c) at ph 8.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 83 |
| Detector technology | CCD |
| Collection date | 2005-05-05 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.770, 93.770, 127.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.370 - 2.400 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.25900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ZFR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.600 |
| Data reduction software | CRYSTAL (CLEAR) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (V1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.010 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.034 | 0.458 |
| Number of reflections | 12161 | |
| <I/σ(I)> | 34.8 | 5.1 |
| Completeness [%] | 89.8 | 92.8 |
| Redundancy | 10.56 | 9.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 293 | PEG2000 MME, LITHIUM SULFATE, SPERMIDINE, COBALT HEXAMMINE, pH 8.60, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG2000 MME | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | LITHIUM SULFATE | ||
| 3 | 1 | 1 | SPERMIDINE | ||
| 4 | 1 | 1 | COBALT | ||
| 5 | 1 | 1 | HEXAMMINE | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | PEG2000 MME | ||
| 8 | 1 | 2 | LITHIUM SULFATE | ||
| 9 | 1 | 2 | COBALT |
