2FGN
Structural Studies Examining the Substrate Specificity Profiles of PC-PLCBc Protein Variants
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2003-12-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 90.522, 90.522, 70.214 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.040 |
| R-factor | 0.22 |
| Rwork | 0.179 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.300 | 2.000 |
| Rmerge | 0.054 | 0.038 | 0.110 |
| Number of reflections | 19621 | ||
| Completeness [%] | 96.5 | 96 | 95.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 295 | 45% saturated ammonium sulfate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
