2FFL

Crystal Structure of Dicer from Giardia intestinalis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-08-26
Detector	ADSC QUANTUM 4
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	155.515, 174.101, 152.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 3.330
R-factor	0.24451
Rwork	0.243
R-free	0.27452
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.143
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.420
High resolution limit [Å]	3.330	3.330
Rmerge	0.114	0.426
Number of reflections	60717
Completeness [%]	99.5	99.3
Redundancy	3.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	28% PEG 400, 0.1M NaCl, 0.1M MnCl2, 0.1M MES, 5 mM TCEP, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	28% PEG 400, 0.1M NaCl, 0.1M MnCl2, 0.1M MES, 5 mM TCEP, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K