2FF4
Mycobacterium tuberculosis EmbR in complex with low affinity phosphopeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-15 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9794 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 162.390, 82.040, 81.080 |
Unit cell angles | 90.00, 115.37, 90.00 |
Refinement procedure
Resolution | 46.000 - 1.900 |
R-factor | 0.21644 |
Rwork | 0.215 |
R-free | 0.23941 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.132 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.600 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.073 | 0.294 |
Number of reflections | 75792 | |
<I/σ(I)> | 15 | 5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1 M Tris-HCl, 2.5% PEG 8000, 3% ethylene glycol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |