2FF3

Crystal structure of Gelsolin domain 1:N-wasp V2 motif hybrid in complex with actin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-18
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9756
Spacegroup name	P 21 21 21
Unit cell lengths	56.996, 69.347, 179.868
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.880 - 2.000
R-factor	0.21218
Rwork	0.210
R-free	0.25010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.052
Data reduction software	HKL-2000
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	89.800
High resolution limit [Å]	1.990
Number of reflections	45279
Completeness [%]	97.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	293.15	5% PEG 8000, 0.1M sodium acetate, 10mM calcium chloride, pH 6.5, microbatch, temperature 293.15K