2FE5
The Crystal Structure of the Second PDZ Domain of Human DLG3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.487, 40.242, 33.340 |
| Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
| Resolution | 26.450 - 1.100 |
| R-factor | 0.11512 |
| Rwork | 0.113 |
| R-free | 0.15038 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2BYG.pdb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.439 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.450 | 1.160 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Number of reflections | 27971 | |
| Completeness [%] | 98.4 | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | Ammonium sulphate, Glycerol, Bis-Tris, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
