2FDN

2[4FE-4S] FERREDOXIN FROM CLOSTRIDIUM ACIDI-URICI

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-08-01
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	33.950, 33.950, 74.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 0.940
R-factor	0.1003
Structure solution method	TAKEN FROM PDB ENTRY 1FDN
RMSD bond length	0.027
RMSD bond angle	0.038
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	SHELX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	0.950
High resolution limit [Å]	0.940	0.940
Rmerge	0.073	0.483
Total number of observations	47452 *
Number of reflections	28084
<I/σ(I)>	15	2
Completeness [%]	98.7	93.7
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7	281 *	55-60% AMMONIUM SULFATE, 100 MM TRISMALEATE PH 6.6-7.0 MIXED 1:1 WITH 10 MG/ML PROTEIN SOLUTION.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	ammonium sulfate	55-60 (%sat)
3	1	drop	TrisMaleate	100 (mM)
4	1	reservoir	ammonium sulfate	55-60 (%sat)
5	1	reservoir	TrisMaleate	100 (mM)