2FDN
2[4FE-4S] FERREDOXIN FROM CLOSTRIDIUM ACIDI-URICI
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-08-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 33.950, 33.950, 74.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.940 |
| R-factor | 0.1003 |
| Structure solution method | TAKEN FROM PDB ENTRY 1FDN |
| RMSD bond length | 0.027 |
| RMSD bond angle | 0.038 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 0.950 |
| High resolution limit [Å] | 0.940 | 0.940 |
| Rmerge | 0.073 | 0.483 |
| Total number of observations | 47452 * | |
| Number of reflections | 28084 | |
| <I/σ(I)> | 15 | 2 |
| Completeness [%] | 98.7 | 93.7 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 281 * | 55-60% AMMONIUM SULFATE, 100 MM TRISMALEATE PH 6.6-7.0 MIXED 1:1 WITH 10 MG/ML PROTEIN SOLUTION. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 55-60 (%sat) | |
| 3 | 1 | drop | TrisMaleate | 100 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 55-60 (%sat) | |
| 5 | 1 | reservoir | TrisMaleate | 100 (mM) |
