2FCZ
HIV-1 DIS kissing-loop in complex with ribostamycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.91946 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 112.125, 27.191, 106.909 |
Unit cell angles | 90.00, 116.72, 90.00 |
Refinement procedure
Resolution | 19.170 - 2.010 |
R-factor | 0.238 |
Rwork | 0.238 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fcx |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 2.010 | 2.010 |
Number of reflections | 18764 | |
<I/σ(I)> | 18.9 | 5.7 |
Completeness [%] | 95.4 | 99.2 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 310 | MPD, KCl, MgCl2, Na Cacodylate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 310K |