2FCP
FERRIC HYDROXAMATE UPTAKE RECEPTOR (FHUA) FROM E.COLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 1998-08-15 |
Detector | MARRESEARCH |
Spacegroup name | P 61 |
Unit cell lengths | 171.550, 171.550, 87.650 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.242 |
Rwork | 0.242 |
R-free | 0.28300 |
Structure solution method | OTHER |
Starting model (for MR) | 1fcp |
RMSD bond length | 0.007 |
RMSD bond angle | 26.400 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.500 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.078 * | 0.334 * |
Number of reflections | 53749 | 2931 * |
<I/σ(I)> | 17 | 2.2 |
Completeness [%] | 99.3 | 92.2 |
Redundancy | 6.4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.4 | 100MM NA-CACODYLATE, PH 6.4, 12.5% PEG2000 MME, 20% GLYCEROL, 1% CIS-INOSITOL, 3% PEG200 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6.5 (mg/ml) | |
2 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.4 |
3 | 1 | reservoir | PEG2000MME | 11 (%) | |
4 | 1 | reservoir | glycerol | 20 (%) | |
5 | 1 | reservoir | PEG200 | 3 (%) | |
6 | 1 | reservoir | dimethyldecylamine-N-oxide | 0.8 (%) | |
7 | 1 | reservoir | cis-inositol | 1 (%) |