2FCB

HUMAN FC GAMMA RECEPTOR IIB ECTODOMAIN (CD32)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	287
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	40.830, 50.920, 80.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.740
R-factor	0.194
Rwork	0.194
R-free	0.27900
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	2.007
Data reduction software	MOSFLM
Data scaling software	CCP4
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000
High resolution limit [Å]	1.740	1.740
Rmerge	0.055
Number of reflections	18009
Completeness [%]	93.0	86.4
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5.4 *	20 *	33% PEG 2000, 0.2M NAOAC, PH5.4, pH 5.3

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	drop	MPS	2 (mM)
3	1	drop		150 (mM)
4	1	drop	sodium azide	0.02 (%)
5	1	reservoir	PEG2000	33 (%)
6	1	reservoir	sodium acetate	0.2 (M)