2FBB

Crystal Structure Analysis of Hexagonal Lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	10
Detector technology	CCD
Collection date	2004-06-30
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	85.637, 85.637, 67.932
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	70.000 - 1.460
R-factor	0.183
Rwork	0.183
R-free	0.19800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1DPW.pdb
RMSD bond length	0.021
RMSD bond angle	1.980
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	1.470
High resolution limit [Å]	1.460	1.460
Rmerge	0.038	0.884
Number of reflections	25570
<I/σ(I)>	77.6	3.5
Completeness [%]	100.0	100
Redundancy	23	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.4	293	10% aqu. acetone solution saturated with sodium nitrate, 25mg/ml lysozyme, pH 8.4, batch, temperature 293.0K, pH 8.40