2FAK
Crystal structure of Salinosporamide A in complex with the yeast 20S proteasome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.168, 301.157, 144.144 |
| Unit cell angles | 90.00, 112.82, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.25400 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.820 |
| Rmerge | 0.467 | |
| Number of reflections | 255532 | |
| Completeness [%] | 100.0 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Crystals appeared after one week; Crystallization conditions: 40mg/ml yeast 20S proteasome 0.1M MES; 28mM MgAc2, 12% MPD 6h crystal soak with 10mM SalA, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
