2FA9

The crystal structure of Sar1[H79G]-GDP provides insight into the coat-controlled GTP hydrolysis in the disassembly of COP II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	CCD
Collection date	2005-08-11
Detector	ENRAF-NONIUS
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	53.215, 61.620, 71.165
Unit cell angles	90.00, 72.75, 90.00

Refinement procedure

Resolution	45.650 - 2.500
R-factor	0.218
Rwork	0.218
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f6b
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	PROTEUM PLUS
Data scaling software	LSCALE
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.000
High resolution limit [Å]	2.500	2.500
Number of reflections	13619
Completeness [%]	88.4	88.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION, RECRYSTALLIZATION	5.8	298	28%(w/v)Polyethylene Glycol-4000, 100mM sodium acetate, 0.2M ammonium sulfate , pH 5.8, EVAPORATION, RECRYSTALLIZATION, temperature 298K