2FA5
The crystal structure of an unliganded multiple antibiotic-resistance repressor (MarR) from Xanthomonas campestris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL12B2 |
Synchrotron site | SPring-8 |
Beamline | BL12B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979575 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 71.420, 71.420, 110.290 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.780 - 1.800 |
Rwork | 0.200 |
R-free | 0.23400 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.780 | |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 52842 | |
Completeness [%] | 90.6 | 90.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 5.5 | 293 | 0.1M NaOAC, 0.2M MgCl2, 22% PEG 2K MME, pH 5.5, LIQUID DIFFUSION, temperature 293K |