2F9I
Crystal Structure of the carboxyltransferase subunit of ACC from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Collection date | 2003-05-25 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.979, 50.859, 149.639 |
| Unit cell angles | 90.00, 113.51, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.980 |
| R-factor | 0.189 |
| Rwork | 0.186 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1on3 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.606 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | DM (4.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.060 | 0.293 |
| Number of reflections | 90018 | |
| Completeness [%] | 97.3 | 82.3 |
| Redundancy | 3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | 30% PEG-400, 0.1M HEPES, pH 8.0, 0.2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
