2F95
M intermediate structure of sensory rhodopsin II/transducer complex in combination with the ground state structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.934 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 124.620, 46.900, 53.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.010 - 2.200 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.24100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 1H2S |
RMSD bond length | 0.007 |
RMSD bond angle | 1.100 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.010 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.085 | 0.414 |
Number of reflections | 16147 | |
Completeness [%] | 97.9 | 99.4 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.8 | 296 | 150 MM NACL, 25 MM NAKPI 5.1 0.8% B-OCTYLGLUCOSID , MONOVACCENIN (CUBIC PHASE) PRECIPITATED BY 1 M NA/KPI 5.8 ,in cubic lipidic phase, temperature 296K |