2F8T
Crystal structure of Aa-Ago with externally-bound siRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.200, 118.077, 98.498 |
| Unit cell angles | 90.00, 99.57, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.100 |
| R-factor | 0.20868 |
| Rwork | 0.204 |
| R-free | 0.29688 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Aa-Ago pdb entry 1YVU |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.722 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.230 |
| High resolution limit [Å] | 3.100 | 3.110 |
| Rmerge | 0.085 | 0.390 |
| Number of reflections | 37080 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEF3350, 25mM NH4F, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEF3350 | ||
| 2 | 1 | 1 | NH4F | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | PEF3350 | ||
| 5 | 1 | 2 | NH4F | ||
| 6 | 1 | 2 | H2O |
