2F8Q
An alkali thermostable F/10 xylanase from alkalophilic Bacillus sp. NG-27
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-03-05 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 174.505, 54.738, 131.497 |
Unit cell angles | 90.00, 131.21, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.197 |
Rwork | 0.197 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hiz |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 46129 | |
Completeness [%] | 96.8 | 86 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 273 | 0.1M NaCl, 0.01M MgCl2, 0.1M Tris pH 8.5 and 18% PEG 8000, VAPOR DIFFUSION, temperature 273K |