2F8N
2.9 Angstrom X-ray structure of hybrid macroH2A nucleosomes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2004-02-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.145, 109.272, 176.273 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.400 - 2.900 |
| Rwork | 0.217 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u35 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.055 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.400 | |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.070 | 0.408 |
| Number of reflections | 44768 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | 34 to 37.5mM KCl and 40-45mM MnCl2, 5mM Potassium Cacodylate, Sample concentration: 8-12 mg/ml, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KCl | ||
| 2 | 1 | 1 | MnCl2 | ||
| 3 | 1 | 1 | Potassium Cacodylate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | KCl | ||
| 6 | 1 | 2 | MnCl2 | ||
| 7 | 1 | 2 | Potassium Cacodylate | ||
| 8 | 1 | 2 | H2O |
