2F7W
Crystal structure of Molybdenum cofactor biosynthesis protein Mog from Shewanella oneidensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 32 |
| Unit cell lengths | 65.910, 65.910, 101.212 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.17979 |
| Rwork | 0.177 |
| R-free | 0.22845 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.501 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.115 | 0.511 |
| Number of reflections | 38213 | |
| <I/σ(I)> | 28.9 | 3.94 |
| Completeness [%] | 98.6 | 98.3 |
| Redundancy | 6.1 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M Ammonium sulfate 0.1 M HEPES pH 7.5 25 % PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
