2F7L
Crystal structure of Sulfolobus tokodaii phosphomannomutase/phosphoglucomutase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-12 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9999 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 161.904, 161.904, 170.494 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.800 |
Rwork | 0.225 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1WQA CHAIN A |
RMSD bond length | 1.627 |
RMSD bond angle | 0.010 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.082 | 0.573 |
Number of reflections | 32892 | |
<I/σ(I)> | 9.4 | 2.19 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 14.5 | 14.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 2.0M ammonium sulfate, 0.1M sodium citrate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |