2F6N

Crystal structure of PHD finger-linker-bromodomain fragment of human BPTF in the free form

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9797
Spacegroup name	P 21 21 2
Unit cell lengths	85.144, 144.894, 38.315
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
Rwork	0.217
R-free	0.23400
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.080
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.105	0.476
Number of reflections	32535
Completeness [%]	99.0	98.1
Redundancy	12.6	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	30% PEG 400, 0.1M Tris 8.5, 0.1M KCl, 10mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K