2F6F

The structure of the S295F mutant of human PTP1B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-D
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-13
Detector	ADSC QUANTUM 4
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	88.330, 88.330, 104.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.186
Rwork	0.183
R-free	0.20300
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1pty
RMSD bond length	0.011
RMSD bond angle	1.450
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNX
Refinement software	CNX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.000	2.000
Number of reflections	31482
<I/σ(I)>	9.1	2.2
Completeness [%]	97.7	87.7
Redundancy	10.1	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	PEG 3350, MGCL2, HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K