2F60
Crystal Structure of the Dihydrolipoamide Dehydrogenase (E3)-Binding Domain of Human E3-Binding Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-12 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 36.845, 36.845, 191.542 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.500 - 1.550 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f5z |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.500 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 12109 | |
| <I/σ(I)> | 67.8 | 8.6 |
| Completeness [%] | 98.9 | 95.4 |
| Redundancy | 13.7 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.3 | 293 | 1.35 M sodium citrate, 0.075% beta-octyl-glucopyranoside, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 6.30 |
