2F60

Crystal Structure of the Dihydrolipoamide Dehydrogenase (E3)-Binding Domain of Human E3-Binding Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-12
Detector	ADSC QUANTUM 315
Spacegroup name	P 65 2 2
Unit cell lengths	36.845, 36.845, 191.542
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.500 - 1.550
R-factor	0.206
Rwork	0.206
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f5z
RMSD bond length	0.033
RMSD bond angle	2.700
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.500	1.610
High resolution limit [Å]	1.550	1.550
Number of reflections	12109
<I/σ(I)>	67.8	8.6
Completeness [%]	98.9	95.4
Redundancy	13.7	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.3	293	1.35 M sodium citrate, 0.075% beta-octyl-glucopyranoside, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 6.30