2F5A

CRYSTAL STRUCTURE OF FAB' FROM THE HIV-1 NEUTRALIZING ANTIBODY 2F5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-C
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	1998-10
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	63.600, 76.400, 94.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.050
Rwork	0.235
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 1CLZ
RMSD bond length	0.009
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.100
High resolution limit [Å]	2.050	2.050
Number of reflections	89376
<I/σ(I)>	15	3.8
Completeness [%]	92.0	93
Redundancy	3.2	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		pH 6.5