2F4Y
Barnase cross-linked with glutaraldehyde
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 2002-03-25 |
Detector | MARRESEARCH |
Wavelength(s) | 0.964 |
Spacegroup name | P 32 |
Unit cell lengths | 58.970, 58.970, 82.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 9.000 - 2.150 |
Rwork | 0.147 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1a2p |
RMSD bond length | 0.005 |
RMSD bond angle | 0.023 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | SHELXL |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 9.000 | 2.500 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 14863 | |
<I/σ(I)> | 16.3 | 7.6 |
Completeness [%] | 93.1 | 92.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 300 | AMMONIUM SULFATE, ZINC CHLORIDE, HEPES BUFFER PROTEIN AT 20MG/ML, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K |