2F4T
Asite RNA + designer antibiotic
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Collection date | 2002-06-01 |
| Wavelength(s) | 0.991 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.976, 32.926, 46.414 |
| Unit cell angles | 90.00, 95.62, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| R-factor | 0.267 |
| Rwork | 0.211 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.440 | 3.000 |
| Rmerge | 0.078 | 0.040 | 0.206 |
| Number of reflections | 2746 | ||
| <I/σ(I)> | 8 | 3.2 | |
| Completeness [%] | 97.7 | 79.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
