2F4O
The Mouse PNGase-HR23 Complex Reveals a Complete Remodulation of the Protein-Protein Interface Compared to its Yeast Orthologs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-05-10 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 96.958, 52.097, 80.848 |
Unit cell angles | 90.00, 113.50, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.260 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2f4m |
RMSD bond length | 0.016 |
RMSD bond angle | 1.559 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.098 | 0.234 |
Number of reflections | 17174 | |
<I/σ(I)> | 18.4 | 2.5 |
Completeness [%] | 91.3 | 49.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 291 | 0.1 M Tris-HCl, 28-32% PEG4000, 0.2 M sodium acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K, pH 8.50 |