2F4F

Crystal structure of IS200 transposase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-06
Detector	ADSC QUANTUM 210
Wavelength(s)	1.07220, 0.97960, 0.97972, 0.97192
Spacegroup name	C 1 2 1
Unit cell lengths	91.930, 67.924, 65.332
Unit cell angles	90.00, 128.73, 90.00

Refinement procedure

Resolution	19.480 - 1.800
R-factor	0.222
Rwork	0.222
R-free	0.23800
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	29289
Completeness [%]	97.9	85.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	297	100mM sodium HEPES (pH 7.5), 25% (w/v) PEG3350, EVAPORATION, temperature 297K