2F4A
Triclinic cross-linked lysozyme soaked with thiourea 1.5M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-03-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.964 |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.280, 32.020, 34.310 |
| Unit cell angles | 88.53, 108.51, 111.92 |
Refinement procedure
| Resolution | 10.000 - 1.950 |
| Rwork | 0.160 |
| R-free | 0.16070 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2f2n |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.022 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.300 | 2.100 |
| High resolution limit [Å] | 1.949 | 1.949 |
| Rmerge | 0.181 | |
| Number of reflections | 6985 | |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 95.5 | 94.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 300 | SODIUM NITRATE AS CRYSTALLIZING AGENT CROSS-LINKED BY VAPOR DIFFUSION WITH GLUTARALDEHYDE, PROTIEN CONCENTRATION 20 MG/ML, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
