2F3Y

Calmodulin/IQ domain complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-10
Detector	MARRESEARCH
Wavelength(s)	0.95144, 1.24242, 0.95056, 0.91838
Spacegroup name	C 1 2 1
Unit cell lengths	86.200, 30.960, 63.940
Unit cell angles	90.00, 114.54, 90.00

Refinement procedure

Resolution	24.370 - 1.450
R-factor	0.189
Rwork	0.189
R-free	0.21900
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	1.000
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.470
High resolution limit [Å]	1.420	1.420
Rmerge	0.042	0.233
Number of reflections	28262
Completeness [%]	96.0	64.1
Redundancy	2.4	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	298	32% PEG 4000, 50mM TRIS, 50 mM MgCl2, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8.3	298	32% PEG 4000, 50mM TRIS, 50 mM MgCl2, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8.3	298	32% PEG 4000, 50mM TRIS, 50 mM MgCl2, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K