2F3X
Crystal structure of FapR (in complex with effector)- a global regulator of fatty acid biosynthesis in B. subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 89.441, 89.441, 162.166 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.250 - 3.100 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.137 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.250 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.079 | 0.310 |
| Number of reflections | 12596 | |
| <I/σ(I)> | 19.3 | 6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 25% ethylenglycol, 5mM malonyl-CoA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
