2F3T
Crystal Structure Of E.coli Guanylate Kinase In Complex With Ganciclovir monophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 107.753, 107.753, 272.663 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 3.160 |
| R-factor | 0.268 |
| Rwork | 0.268 |
| R-free | 0.29000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2anc |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.360 |
| High resolution limit [Å] | 3.160 | 3.160 |
| Rmerge | 0.122 | 0.452 |
| Number of reflections | 28251 | |
| <I/σ(I)> | 20.1 | 7.7 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 16.4 | 16.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 13% (w/v) PEG 3350, 80 mM Hepes, 100 mM sodium cacodylate and 20 mM betaine, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
