2F3L
Crystal Structure of a Lumenal Rfr-domain protein (Contig83.1_1_243_746) from Cyanothece sp. 51142 at 2.1 Angstrom resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 61.754, 61.754, 83.708 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.110 |
R-factor | 0.2168 |
Rwork | 0.217 |
R-free | 0.25080 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.003 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.110 | 2.110 |
Rmerge | 0.076 | 0.218 |
Number of reflections | 9861 | |
<I/σ(I)> | 25.2 | 3.56 |
Completeness [%] | 90.0 | 57.1 |
Redundancy | 6.2 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 295 | 15% PEG 1500, 50 mM sodium chloride, 10 mM Tris, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 295 | 15% PEG 1500, 50 mM sodium chloride, 10 mM Tris, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 295K |