2F3G

IIAGLC CRYSTAL FORM III

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1993-06
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 21 21
Unit cell lengths	60.040, 70.230, 74.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.130
R-factor	0.169 *
Rwork	0.169
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f3g
RMSD bond length	0.011
RMSD bond angle	21.000 *
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	UNPUBLISHED
Refinement software	TNT (5E)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.130
Rmerge	0.036
Total number of observations	44614 *
Number of reflections	15616
Completeness [%]	86.0
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5		30% PEG 4000, 0.2M MGCL2, 0.1M TRIS, PH 8.5 4 DEGREES

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4 (mg/ml)
2	1	reservoir	mPEG4000	30 (%)
3	1	reservoir		0.2 (M)
4	1	reservoir	Tris-HCl	0.1 (M)