2F31
Crystal structure of the autoinhibitory switch in Formin mDia1; the DID/DAD complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 200 |
| Detector technology | AREA DETECTOR |
| Collection date | 2005-05-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54180 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.160, 65.790, 46.860 |
| Unit cell angles | 90.00, 104.08, 90.00 |
Refinement procedure
| Resolution | 7.970 - 2.100 |
| R-factor | 0.2328 |
| Rwork | 0.229 |
| R-free | 0.31170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.556 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.200 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.058 | 0.347 |
| Number of reflections | 16811 | |
| <I/σ(I)> | 11.54 | 2.93 |
| Completeness [%] | 84.8 | 83.6 |
| Redundancy | 2.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M sodium citrate, 200 mM ammonium sulfate, 25% PEG 4000, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
